Figure 1
The Prostaglandin pathway: expression and abundance of the prostaglandin metabolites synthesized by Skeletonema marinoi FE7 and FE60 strains. (a) Schematic representation of prostaglandin synthesis pathway, starting from the three main precursors: eicosatrienoic, arachidonic and eicosapentaenoic acids. The most abundant precursor PUFAs in diatoms is shown in red. Square box contains the prostaglandins identified in our study in relation to their respective PUFA precursors. Colored rectangles or ovals indicate presence of each enzyme in the annotation table of Skeletonema marinoi strains FE7 or FE60 (see legend). (b–d) Expression levels of enzymes responsible for PGs synthesis and of the prostaglandin transporter measured by Real-time qPCR in exponential, stationary and senescent phases of growth. Results were analysed with the REST software and reported as 2 log expression ratio in the exponential phase (control) ±s.d. (b, FE7 strain; c, FE60 strain) or as 2 log expression ratio of FE60 with respect to FE7 strain (d). Statistical analysis (N=3) was performed using the Pair Wise Fixed Reallocation Randomization test by REST. Relative expression ratios above two fold were considered significant. (e) Abundance of the prostaglandin metabolites identified in our Skeletonema marinoi strains, quantified with LC/MSMS in the three different phases of growth (N=3). Results are means of three technical replicates (b–e) for each biological replicate (N=3).
