Abstract
In the course of our screening for antitrypanosomal compounds from soil microorganisms, as well as from the antibiotics library of the Kitasato Institute for Life Sciences, we found three peptide antibiotics, leucinostatin (A and B), alamethicin I and tsushimycin, which exhibited potent or moderate antitrypanosomal activity. We report here the in vitro and in vivo antitrypanosomal properties and cytotoxicities of leucinostatin A and B, alamethicin I and tsushimycin compared with suramin. We also discuss their possible mode of action. This is the first report of in vitro and in vivo trypanocidal activity of leucinostatin A and B, alamethicin I and tsushimycin.
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Acknowledgements
This work was supported, in part, by funds from the Drugs for Neglected Diseases initiative (DNDi), and a grant for All Kitasato Project Study (AKPS). We are grateful to Dr Y Yabu, the Nagoya City University, Professor R Brun and Dr M Kaiser, the Swiss Tropical Institute, Professor T Kinoshita, the Research Institute for Microbial Diseases, Osaka University and Professor S Croft, Dr Eric Chatelain, Dr J-R Ioset, Dr C Brünger and Miss F Hirabayashi, DNDi, for valuable discussions. We also thank Miss H Sekiguchi, Mr T Furusawa, Miss M Niitsuma and Miss J Hashida (Kitasato university) for her technical assistance throughout this work.
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Ishiyama, A., Otoguro, K., Iwatsuki, M. et al. In vitro and in vivo antitrypanosomal activities of three peptide antibiotics: leucinostatin A and B, alamethicin I and tsushimycin. J Antibiot 62, 303–308 (2009). https://doi.org/10.1038/ja.2009.32
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DOI: https://doi.org/10.1038/ja.2009.32
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