Abstract
No enzyme systems responsible for the biosynthesis of structurally and biosynthetically intriguing bis-5-alkylresorcinols produced by plants have been identified. Herein, we show that bacterial, fungal and plant alkylresorcinol-producing type III polyketide synthases (PKSs), such as ArsB in the Gram-negative bacterium Azotobacter vinelandii, ORAS in the fungus Neurospora crassa and ARAS2 in the rice plant Oryza sativa, can synthesize bis-5-alkylresorcinol from alkanedioic acid N-acetylcysteamine dithioester as a starter substrate and from malonyl-CoA as an extender substrate by two-step conversion. Plants presumably use a type III PKS for the biosynthesis of bis-5-alkylresorcinols.
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Acknowledgements
This work was supported by a research grant from the New Energy and Industrial Technology Development Organization of Japan and a Grant-in-Aid for Scientific Research on Priority Area ‘Applied Genomics’ from Monkasho.
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Miyanaga, A., Horinouchi, S. Enzymatic synthesis of bis-5-alkylresorcinols by resorcinol-producing type III polyketide synthases. J Antibiot 62, 371–376 (2009). https://doi.org/10.1038/ja.2009.44
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DOI: https://doi.org/10.1038/ja.2009.44
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