Abstract
Piceamycin, a new macrolactam polyketide antibiotic, was detected by HPLC-diode array screening in extracts of Streptomyces sp. GB 4-2, which was isolated from the mycorrhizosphere of Norway spruce. The structure of piceamycin was determined by mass spectrometry and NMR experiments. It showed inhibitory activity against Gram-positive bacteria, selected human tumor cell lines and protein tyrosine phosphatase 1B.
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Acknowledgements
Financial support from the Deutsche Forschungsgemeinschaft (Graduate College 685 ‘Infection Biology’; DS, JR), the European Commission (project ACTINOGEN, 6th framework, Grant LSHM-CT-2004-005224; RDS), and Bayer Schering Pharma AG (Berlin, Germany) is gratefully acknowledged. We thank Dr Thomas Paululat, Universität Siegen, for helpful discussions and for performing the CIGAR-NMR experiments, and Ms Gaby Biegert, Universität Tübingen, for isolation of streptomycetes strains from rhizospheric soils.
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Art. no. 50 in ‘Biosynthetic Capacities of Actinomycetes’. Art. no. 49: see Hohmann et al.
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Schulz, D., Nachtigall, J., Riedlinger, J. et al. Piceamycin and its N-acetylcysteine adduct is produced by Streptomyces sp. GB 4-2. J Antibiot 62, 513–518 (2009). https://doi.org/10.1038/ja.2009.64
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DOI: https://doi.org/10.1038/ja.2009.64
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