Abstract
An actinomycete strain, IR73-Li102T, was isolated from a lichen sample obtained from Iriomote Island, Japan, and subsequently characterized using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain IR73-Li102T had the highest sequence similarities with Actinomycetospora chiangmaiensis YIM 0006T (98.3%), A. corticola 014-5T (98.1%) and A. rishiriensis RI109-Li102T (98.0%). However, DNA–DNA hybridization assays, as well as physiological and biochemical analyzes, showed that strain IR73-Li102T could be clearly differentiated from its closest phylogenetic relatives. The strain contained meso-diaminopimelic acid, and arabinose and galactose were present in whole-cell hydrolysates. The predominant menaquinone was MK-8(H4), and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acid was iso-C16:0 (58%). The chemotaxonomic properties of strain IR73-Li102T were consistent with those shared by members of the genus Actinomycetospora. On the basis of the phenotypic features and DNA–DNA hybridization data, strain IR73-Li102T (= NBRC 106365T = KCTC 19783T) represents a novel species of the genus Actinomycetospora, for which the name Actinomycetospora iriomotensis sp. nov. is proposed.
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Acknowledgements
We are grateful to Mr Yuya Sakuraki for technical assistance. This study was supported in part by a research grant from the Institute for Fermentation, Osaka (IFO), Japan. We are grateful to Dr Jean P Euzéby for support with nomenclature.
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Yamamura, H., Ashizawa, H., Nakagawa, Y. et al. Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample. J Antibiot 64, 289–292 (2011). https://doi.org/10.1038/ja.2011.15
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DOI: https://doi.org/10.1038/ja.2011.15
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