Abstract
Four germicidin homologs were isolated from a liquid culture of Streptomyces coelicolor A3(2). These were identified as germicidins A, B and C, and surugapyrone A (germicidin D). Absolute stereochemistry of the chiral center in germicidins A and C is determined to be S. All germicidins inhibited germination of S. coelicolor A3(2) spores above 1 μg ml−1. S. coelicolor A3(2) spores collected from a single petri dish (9 cm i.d.) contained 5.4 μg of germicidin A (∼2.7 × 10−14 g per spore), which accounts for 2.3% of the spore extract, and contents of germicidins B, C and D were 0.2–0.8 μg. The activity of the spore extract corresponded well with the sum of the activity of each germicidin, which was estimated from the content and dose–response curve, which indicates that germicidins functions as self-germination inhibitors in S. coelicolor A3(2). Inhibitory action of germicidin A on spore germination was reversible and germicidin A inhibited not only spore germination but also hyphal elongation.
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Acknowledgements
This study was supported, in part, by Grant-in-Aid for Scientific Research (C) from Japan Society for the Promotion of Science (No. 20580109).
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Aoki, Y., Matsumoto, D., Kawaide, H. et al. Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2). J Antibiot 64, 607–611 (2011). https://doi.org/10.1038/ja.2011.59
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DOI: https://doi.org/10.1038/ja.2011.59
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