Abstract
A new polyene macrolide family, closely related to the pentaene macrolide antibiotic roflamycoin, was isolated from the both fermentation broth and biomass of Streptomyces durmitorensis wild-type strain MS405. The main compound was identified by NMR and Fourier transform ion cyclotron resonance mass spectrometry as 32,33-didehydroroflamycoin (1; DDHR). Additional four structurally related compounds were determined solely by MS analysis. DDHR induces cell death by apoptosis in various cancer cell lines as demonstrated by DNA fragmentation. Striking feature of DDHR is its internal fluorescence allowing visualization of labeled plasma membranes and internal membrane structures.
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Acknowledgements
We thanks the Institutional Research Concept AV 0Z502 00510, Center of Molecular and Cellular Immunology 1M6837805001 nda MSM0021620858, grant no. 2B08064 of the Ministry of Education Youth and Sport, grant no. 143056 of the Ministry of Science of the Republic of Serbia and Bilateral cooperation project between Serbian Academy of Sciences and Academy of Sciences of the Czech Republic for their financial contribution.
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Stodůlková, E., Kuzma, M., Hench, I. et al. New polyene macrolide family produced by submerged culture of Streptomyces durmitorensis. J Antibiot 64, 717–722 (2011). https://doi.org/10.1038/ja.2011.81
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DOI: https://doi.org/10.1038/ja.2011.81
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