Abstract
Three new phthalide derivatives (1-3) named 5-(3′-methyl-2′-butenyl)-2-hydroxy-3-methoxy-4-methylbenzoic acid (1), 5-(3′-carboxyl-3′-methyl-2E-allyloxy)-3-methoxy-4-methylphthalide (2) and 5-(3′,3′-dimethylallyloxy)-2-methoxycarbonyl-3-methoxy-4-methylbenzoic acid (3) together with six known phthalide derivatives named 5-(3′,3′-dimethylallyloxy)-3-methoxy-4-methylphthalide (4), zinnimidine (5), 5-(3′,3′-dimethylallyloxy)-3-methoxy-4-methylphthalide (6), 5-(3′,3′-dimethylallyloxy)-3-methoxy-4-methylphthalic acid (7), zinniol anhydride (8) and porriolide (9) were isolated from the liquid culture of the plant endophytic fungus Pestalotiopsis photiniae isolated from the Chinese Podocarpaceae plant Podocarpus macrophyllus. Their structures were elucidated by extensive spectroscopic analysis. Compounds 1–9 displayed significant antifungal activities against three plant pathogens.
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Acknowledgements
This work was supported by the programs for New Century Excellent Talents in University (NCET-09-0112), the Key Project of Chinese Ministry of Education and the Key Applied Basic Research Programs of Hebei Province (0996030917D) and National Natural Science Foundation of China (31071701), Hebei Province Science Fund for Distinguished Young Scholars (C2011201113), National Department Public Benefit Research Foundation (201003065) and Project supported by the National High Technology Research and Development Program of China (Grant no. 2011AA10A204).
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Yang, XL., Zhang, S., Hu, QB. et al. Phthalide derivatives with antifungal activities against the plant pathogens isolated from the liquid culture of Pestalotiopsis photiniae. J Antibiot 64, 723–727 (2011). https://doi.org/10.1038/ja.2011.82
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DOI: https://doi.org/10.1038/ja.2011.82
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