Figure 3 | The Journal of Antibiotics

Figure 3

From: Pre-clinical pharmacokinetics and anti-chlamydial activity of salicylidene acylhydrazide inhibitors of bacterial type III secretion

Figure 3

Accumulation of the auto fluorescent compound ME0192 in C. trachomatis-infected HeLa cells. Chlamydia inclusions, indicated by arrows, can be distinguished by their morphology in nucleic acid staining (b) and (e) (SYTOX orange) and (h) (DAPI). (a–c) Cells incubated with ME0192. The blue fluorescent ME0192 accumulates in Chlamydia inclusions (a). (c) is a merge of (a) and (b). (d-f): Cells incubated with solvent (1% DMSO), only background in the blue channel (d). (f) is a merge of (d) and (e). (g–k) Immunostaining: green Chlamydia inclusions (g), DAPI (h), Evans blue staining of cells (i) and merge (k).

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