Abstract
Three new natural products, designated trehangelins A, B and C, were isolated by solvent extraction, silica gel and octadecylsilyl silica gel column chromatographies and subsequent preparative HPLC from the cultured broth of an endophytic actinomycete strain, Polymorphospora rubra K07-0510. The trehangelins consisted of a trehalose moiety and two angelic acid moieties. Trehangelins A (IC50 value, 0.1 mg ml−1) and C (IC50 value, 0.4 mg ml−1), with symmetric structures, showed potent inhibitory activity against hemolysis of red blood cells induced by light-activated pheophorbide a. However, trehangelin B, with an asymmetric structure, displayed only a slight inhibition (IC50 value, 1.0 mg ml−1).
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Acknowledgements
This work was supported, in part, by funds from Quality Assurance Flamework of Higher Education from the Ministry of Education, Culture, Sports, Sciences and Technology (MEXT), Japan. We are grateful to Mr Toru Kimura in our laboratory for his technical assistance, Ms Noriko Sato of Kitasato University for measurements of NMR spectra and Dr Tomoyasu Hirose for advice on the structure.
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Nakashima, T., Okuyama, R., Kamiya, Y. et al. Trehangelins A, B and C, novel photo-oxidative hemolysis inhibitors produced by an endophytic actinomycete, Polymorphospora rubra K07-0510. J Antibiot 66, 311–317 (2013). https://doi.org/10.1038/ja.2013.17
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DOI: https://doi.org/10.1038/ja.2013.17
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