Two new pentanorlanostane metabolites from a soil fungus Curvularia borreriae strain HS-FG-237

The soil fungus C. borreriae strain HS-FG-237 was isolated from samples of loess taken from vegetable plots collected from Xiangtan of Hunan Province, China. It was provided and identified as C. borreriae by the professor Tianyu Zhang at the Shandong Agricultural University, China. The strain HS-FG-237 has been deposited in the Pharmaceutical Research Culture Collection, Zhejiang Hisun Group Co., Ltd, with accession No.: HS-FG-237.

The working strain was grown and preserved on Potato Dextrose Agar (PDA) slant and incubated under static conditions at 24 °C for 6–7 days. The stock culture was transferred into 1-liter Erlenmeyer flasks containing 250 ml of the seed medium consisting of potato starch (Huling, Huzhou Zhanwang Parmaceutical Co., Ltd, Zhejiang, China) 3%, glucose (Sinopharm Chemical Reagent Co., Ltd, Shanghai, China) 4%, peptone (Sincere, Shanghai Sincere Biotech Co. Ltd, Shanghai, China) 0.4%, MgSO₄·7H₂O (Sinopharm Chemical Reagent Co., Ltd) 0.1%, KH₂PO₄ (Sinopharm Chemical Reagent Co., Ltd) 0.2% (natural pH) and incubated at 24 °C for 48 h, shaken at 250 r.p.m. Then, 1 liter of the culture was transferred into a 50-liter fermentor (containing 30-liter fermentation broth), which was composed of peptone 0.5%, potato starch 0.5%, yeast extract (Oxoid Ltd, Basingstoke, Hampshire, UK) 0.2%, NaCl (Sinopharm Chemical Reagent Co., Ltd) 0.4%, KH₂PO₄ 0.1%, MgSO₄·7H₂O 0.05%, CaCO₃ (Sinopharm Chemical Reagent Co., Ltd) 0.2% (pH 6.2–6.4). The fermentation was carried out at 24 °C for 7 days stirred at 100 r.p.m. with an aeration rate of 900 liters of air per hour.

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