Abstract
Natural products still continue to have an important role as a resource of various biologically active substances. Dereplication is a key process in natural product screening that analyzes the extracts of microbial fermentation broths or plant samples. In this review article, we describe and discuss the analytical techniques of dereplication and related technologies in the following sections: 1. Direct detection from microbial colonies. 2. Ultra high performance liquid chromatography (UHPLC)-MS profiling for library construction. 3. Micro-fractionation to identify active peaks. 4. Quantification of small-amount compounds. 5. Structure identification from small amounts. Using these techniques, the desired compound in the mixture library can be rapidly identified.
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Acknowledgements
We thank Professor Yoshikazu Takahashi for giving us the opportunity to prepare this review. We also thank Drs Hideki Sato, Tetsuo Kojima, Takamichi Odake, Yoshie Nagahashi and Hideyuki Katoh for helpful discussion. Editing support was provided by Chugai Editing Services at Chugai Pharmaceutical Co., Ltd.
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Ito, T., Masubuchi, M. Dereplication of microbial extracts and related analytical technologies. J Antibiot 67, 353–360 (2014). https://doi.org/10.1038/ja.2014.12
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DOI: https://doi.org/10.1038/ja.2014.12
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