Abstract
Two novel Gram-stain-positive actinobacteria, designated H23-8T and H23-19, were isolated from a sea sediment sample and their taxonomic positions were investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that these isolates were closely related to the members of the genus Agromyces, with similarity range of 94.5–97.4%. Strains H23-8T and H23-19 contained L-2,4-diaminobutyric acid, D-alanine, D-glutamic acid and glycine in their peptidoglycan. The predominant menaquinones were MK-13 and MK-12, and the major fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The DNA G+C content was 72.3–72.5 mol%. The chemotaxonomic characteristics of the isolates matched those described for members of the genus Agromyces. The results of phylogenetic analysis and DNA–DNA hybridization, along with differences in phenotypic characteristics between strains H23-8T and H23-19 and the species of the genus Agromyces with validly published names, indicated that the two isolates should be assigned to a novel species of the genus Agromyces, for which the name Agromyces marinus sp. nov. is proposed; the type strain is H23-8T (=NBRC 109019T=DSM 26151T).
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Hamada, M., Shibata, C., Tamura, T. et al. Agromyces marinus sp. nov., a novel actinobacterium isolated from sea sediment. J Antibiot 67, 703–706 (2014). https://doi.org/10.1038/ja.2014.60
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DOI: https://doi.org/10.1038/ja.2014.60
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