Figure 1 | The Journal of Antibiotics

Figure 1

From: Linezolid, vancomycin and daptomycin modulate cytokine production, Toll-like receptors and phagocytosis in a human in vitro model of sepsis

Figure 1

Linezolid (LIN), vancomycin (VAN) and daptomycin (DAP) differentially modulate the expression of cytokines in monocytes. THP-1 monocytes were incubated with LIN, VAN or daptomycin in the presence or absence of lipopolysaccharide (LPS) for 24 h. Quantitative real-time-PCR (qRT-PCR) analysis was performed to determine the expression levels of genes encoding TNFα (a), IL-1β (b), IL-6 (c) and IL-10 (d) after normalization to GAPDH. Each bar represents the fold change in mRNA expression comparing unstimulated control with antibiotic-incubated cells or LPS-stimulated control with LPS plus antibiotic-stimulated cells, respectively. Data are expressed as mean±s.e.m. of six independent experiments; *P<0.05 vs respective LPS-free control; +P<0.05 vs respective LPS control.

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