Abstract
A new compound designated as clonoamide was isolated from a culture broth of the fungus Clonostachys sp. BF-0131 by solvent extraction, Diaion HP20 column chromatography, octadecylsilyl column chromatography and preparative HPLC as an inhibitor of sterol O-acyltransferase (SOAT). The structure of clonoamide was elucidated as 2-oxo-9E,11E-tridecandienyl acetamide by various spectral analyses including NMR. The compound inhibited SOAT1 and SOAT2 isozymes with IC50 values of 39 and 110 μm, respectively, in a cell-based assay using SOAT1- and SOAT2-expressing Chinese hamster ovary cells.
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Acknowledgements
We wish to thank Ms Noriko Sato and Dr Kenichiro Nagai (School of Pharmaceutical Sciences, Kitasato University) for measurements of NMR spectra and MS data. This work was supported by a Grant-in-aid for Scientific Research (A) 26253009 from the Ministry of Education, Culture, Sports, Science and Technology, Japan (to HT).
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Kobayashi, K., Tsukasaki, N., Uchida, R. et al. Clonoamide, a new inhibitor of sterol O-acyltransferase, produced by Clonostachys sp. BF-0131. J Antibiot 68, 615–619 (2015). https://doi.org/10.1038/ja.2015.37
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DOI: https://doi.org/10.1038/ja.2015.37
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