Strain Streptomyces sp. HS-NF-1006 was isolated from a soil sample collected from a pasture, located in Hailaer, Inner Mongolia Autonomous Region, China. The strain was identified as the genus Streptomyces because its 16S rRNA sequence (accession no: KU848003) exhibited a high sequence similarity of 99.45% with that of Streptomyces rishiriensis strain NBRC13407 (accession no: AB184383) and it was deposited in the Pharmaceutical Research Culture Collection, Zhejiang Hisun Group Co., Ltd., with accession no: HS-NF-1006.

This strain was incubated for 6–7 days at 28 °C on the medium containing glucose (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) 4 g, malt extract (Bei Jing Ao Bo Xing, Beijing, China) 10 g, yeast extract (Oxoid Ltd, Basingstoke, UK) 4 g, CoCl2·6H2O 0.005 g and agar (Becton, Dickinson and company, Franklin Lake, NJ, USA) 18 g in 1.0 l of water, pH 7.0–7.2. The seed medium consisted of glucose 4 g, malt extract 10 g, yeast extract 4 g and CaCO3 2 g in 1.0 l water, pH 7.2–7.4. All the media were sterilized at 121 °C for 20 min. Fermentation was carried out in a 50 l fermentor (containing 30 l of production medium; Shanghai Guoqiang Bioengineering Equipment, Shanghai, China). The producing medium was composed of glucose 1%, soluble starch (Haiyan Liuhe Starch Chemical Co, Ltd., Haiyan, China) 4%, yeast extract 0.4%, malt extract 1%, CaCO3 0.2%, FeSO4·7H2O 0.1%, ZnSO4·7H2O 0.1%, and MnCl2·4H2O 0.1% at pH 7.2–7.4 before sterilization. The fermentation was conducted at 28 °C for 7 days by stirring at 200 r.p.m. with an aeration rate of 1000 l of air per hour.

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