Table 1 Substrate specificity
From: Amide compound synthesis by adenylation domain of bacillibactin synthetase
Substrate | Specific activity (units mg−1) | Relative activity (%) |
|---|---|---|
DHB (2,3-dihydroxybenzoic acid) | 0.00574 | 100 |
2-Hydroxybenzoic acid | 0.00385 | 67 |
3-Hydroxybenzoic acid | 0.00132 | 23 |
4-Hydroxybenzoic acid | ND | |
Benzoic acid | 0.00115 | 20 |
3-Methylbenzoic acid | ND | |
4-Methylbenzoic acid | ND | |
3-Aminobenzoic acid | ND | |
4-Aminobenzoic acid | ND | |
3-Cyanobenzoic acid | ND | |
4-Cyanobenzoic acid | ND | |
3-Nitorbenzoic acid | ND | |
4-Nitorbenzoic acid | ND | |
4-Chlorobenzoic acid | ND | |
2,6-Difluorobenzoic acid | ND | |
Phthalic acid | ND | |
Cinnamic acid | ND | |
Propionic acid | ND | |
Butyric acid | ND | |
Pentanoic acid | 0.00060 | 10 |
Hexanoic acid | ND | |
Heptanoic acid | 0.00041 | 7.1 |
Octanoic acid | 0.00047 | 8.2 |
Nonanoic acid | ND |
