Abstract
A new depsidone, named 7-chlorofolipastatin, and five known structurally related depsidones were isolated from the culture broth of the marine-derived fungus Aspergillus ungui NKM-007 by solvent extraction and HPLC using an octadecylsilyl column. The structure of 7-chlorofolipastatin was elucidated by various spectroscopic data including 1D and 2D NMR spectroscopy. 7-Chlorofolipastatin inhibited sterol O-acyltransferase (SOAT) 1 and 2 isozymes in cell-based and enzyme assays using SOAT1- and SOAT2-expressing Chinese hamster ovary (CHO) cells.
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Acknowledgements
We thank Ms N Sato and Dr K Nagai, School of Pharmacy, Kitasato University, for measurements of mass and NMR spectra. This work was supported by JSPS KAKENHI Grant Number 26253009 (to HT).
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Uchida, R., Nakajyo, K., Kobayashi, K. et al. 7-Chlorofolipastatin, an inhibitor of sterol O-acyltransferase, produced by marine-derived Aspergillus ungui NKH-007. J Antibiot 69, 647–651 (2016). https://doi.org/10.1038/ja.2016.27
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DOI: https://doi.org/10.1038/ja.2016.27
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