Figure 2
Effect of oral administration of Ramizol on survival rate in the hamster C. difficile (strain BAA-1805, 027 ribotype) -induced colitis. The animals were orally inoculated with C. difficile (BAA- 1805) at a lethal LD90-100 inoculum size (1.35 × 105 spores per animal) in 0.5 ml PBS. Test substance was orally administered twice daily for 5 days starting 16 h after infection. Mortality was monitored for 28 days for all groups. The Fisher’s exact test was used to determine the statistical significance compared with the untreated vehicle group at *P<0.05. Inset table shows the mean log (spore counts) in hamsters (n=7) at the conclusion of the study for each study group. Effect of oral administration of Ramizol and vancomycin on fecal spore counts on day 3 in the hamsters infected with C. difficile strain BAA-1805, an 027 ribotype strain. The animals were orally inoculated with C. difficile (BAA-1805) at an LD90-100 inoculum size (1.43 × 105 spores per animal) in 0.5 ml PBS. Test substance was orally administered twice daily for 5 days starting 16 h after infection. The feces were collected on day 3 (40–64 h post infection) from surviving animals. A 2-log reduction (or more) in the spore counts of the treatment groups compared with the vehicle group indicated significant activity presented as (*). Fecal spore counts below the limit of detection (1.54) were indicated by % clearance. Vehicle is 2% polysorbate 80/0.9% NaCl. LOD, limit of detection.
