Abstract
An in vitro study aimed to evaluate the effect of N-acetyl cysteine (NAC) or sub-ICs of erythromycin on antimicrobial susceptibility of staphylococcal biofilms was performed. Staphylococcus aureus and Staphylococcus epidermidis strains were isolated from patients with prosthetic joint infections using a previously published sonication procedure. Conventional susceptibility studies were performed using microdilution according to the CLSI procedures. Biofilm susceptibility was performed using the Calgary methodology. The addition of NAC showed no effect with the S. aureus strains, and a strain-dependent effect with the S. epidermidis strains. No effect was detected with erythromycin for almost all tested strains.
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Acknowledgements
This work was funded by a grant from the Spanish Ministry of Science (FUNCOATCSD2008-00023) and the Spanish MINECO (MAT2013-48224-C2-2-R).
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JE and RF-R have received travel grants from Pfizer, Novartis, bioMérieux and Alere and research grants from Wyeth and Laboratorios Leti. The remaining authors declare no conflict of interest.
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Molina-Manso, D., Del-Prado, G., Gómez-Barrena, E. et al. Effect of different agents with potential antibiofilm activity on antimicrobial susceptibility of biofilms formed by Staphylococcus spp. isolated from implant-related infections. J Antibiot 69, 686–688 (2016). https://doi.org/10.1038/ja.2016.9
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DOI: https://doi.org/10.1038/ja.2016.9
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