Table 2 6-dEB production in wild-type S. coelicolor PCC and engineered S. coelicolor and M. fulvus PCC strains.
Experiment | PCC homolog | β-subunit present | α-subunit present | Codon usage | β-subunit 5′UTR | β-subunit N-terminal tag | β-subunit C-terminal tag | α-subunit 5′UTR | α-subunit N-terminal tag | α-subunit C-terminal tag | 6-dEB (mg l–1) |
|---|---|---|---|---|---|---|---|---|---|---|---|
1 | S. coelicolor | Yes | Yes | wild-type | 5′UTR-B | N1 | — | 5′UTR-Sc | — | — | 20 ± 3 |
2 | S. coelicolor | Yes | Yes | wild-type | 5′UTR-B | N1 | — | 5′UTR-Sc | — | C2 | 8±2 |
3 | S. coelicolor | Yes | Yes | engineered | 5′UTR-B | N2 | C1 | 5′UTR-A | N3 | C3 | 3.0±0.4 |
4 | S. coelicolor | Yes | Yes | engineered | 5′UTR-B | N2 | — | 5′UTR-A | — | C3 | 20±2 |
5 | M. fulvus | Yes | Yes | engineered | 5′UTR-B | N2 | C1 | 5′UTR-A | N3 | C3 | 6±1 |
6 | M. fulvus | Yes | Yes | engineered | 5′UTR-B | N2 | — | 5′UTR-A | — | C3 | 10.5±0.2 |
7 | S. coelicolor | No | Yes | engineered | — | — | — | 5′UTR-A | N3 | C3 | 2.2± 0.7 |
8 | S. coelicolor | Yes | No | engineered | 5′UTR-B | N2 | C1 | — | — | — | 4±1 |
9 | No PCC | No | No | — | — | — | — | — | — | — | 0.5±0.3 |
