Summary
Sister chromatid exchange (SCE) was examined in cultured lymphocytes of nephrotic children and some other juvenile patients treated with immunosuppressive agents, azathioprine (AZA) and cyclophosphamide (CYC), and CYC in combination with a protein-bound polysaccharide (PS-K). The frequency of SCEs in children treated with CYC showed a highly significant increase as compared with the non-treated control. AZA treatment also resulted in an increased frequency of SCEs, though the mean value was lower than that obtained for CYC. An extremely high frequency of SCEs resulted from a combined treatment with AZA and CYC. After 3–4 months of cessation of CYC medication, the frequency of SCEs returned to the normal level. The SCE frequency decreased significantly when CYC was used in combination with PS-K.
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References
Cahen, R.L. 1966. Experimental and clinical chemoteratogenesis.Advances in Pharmacology. S. Garattini, et al. ed., Academic Press New York, Vol. 4, pp. 263–349.
Chaube, S., and Murphy, M.L. 1968. The teratogenic effects of the recent drugs active in cancer chemotherapy.Advance in Teratology. D.H.M. Woollam, ed., Logos Press, London, Vol. 3, pp. 181–237.
Dobos, M., Schuler, D., and Fekete, Gy. 1974. Cyclophosphamide-induced chromosomal aberrations in nontumorous patients.Humangenetik. 22:221–227.
Ganner, E., Osment, J., Dittrich, P., and Huber, H. 1973. Chromosomes in patients treated with azathioprine.Humangenetik. 18:231–236.
Greenberg, L.H., and Tanaka, K.R. 1964. Congenital anomalies probably induced by cyclophosphamide.JAMA. 188:423–426.
Kureha Chemical Industry Co., Ltd. 1977. Toxicity and genetic test,Outline of PS-K. Kureha Chemical Industry Co., Ltd., Tokyo, pp. 31–33.
Manny, N., Rosenman, E., and Benbassat, J. 1972. Hazard of immunosuppressive therapy.Brit. Med. J. ii:291.
Nakao, Y., Taguchi, T., Shiba, S., and Arakawa, Y. 1973. Influence of protein polysaccharide (PS-K) isolated fromBasidiomycetes on delayed hypersensitivity in sarcome-180 bearing mice.Proc. Jpn. Cancer Assoc. 32:282.
Nomoto, K., Yoshikumi, C., Matsunaga, K., Fujii, T., and Takeya, K. 1975. Restoration of antibody-forming capacities by PS-K in tumor-bearing mice.Gann. 66:365–374.
Ohono, R., Yokomaki, S., Wakayama, K., Sugiura, S., and Yamada, K., 1976. Blastoid transformation of human lymphocytes cultured with protein-bound polysaccharide preparation, PS-K,in vitro.Gann. 67:713–716.
Perry, P., and Evans, H.J. 1975. Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange.Nature 258:121–125.
Rosenkrantz, J.G., Githens, J.H., Cox, S.M., and Kellum, D.L. 1967. Azathioprine (Imuran) and pregnancy.Am. J. Obest. Gynecol. 97:387–394.
Seto, T., Matsuki, M., Saito, K., Arakawa, Y., Fujii, T., Ueno, S., and Hotta, T. 1975a. Studies on teratogenic activity of PS-K (I). Effects of PS-K pre-and post-natal development of mice and rats.The clinical report 9:7–35.
Seto, T., Matsuki, M., Saito, K., Arakawa, Y., Fujii, T., Ueno, S., and Hotta, T. 1975b. Studies on teratogenic activity of PS-K (II). Effects of PS-K on pre- and post-natal developments in the second (F2) of mice and rats.The clinical report 9:38–65.
Tallent, M.B., Simmons, R.L., and Najarian, J.S. 1970. Birth defects in child of male recipient of kidney transplant.JAMA. 211:1854–1855.
Torigoe, K. 1979. Sister chromatid exchange in children treated with anticonvulsant drugs.Acta Med. Biol. 27:65–72.
Tsukagoshi, S. 1974. Host-mediated antitumor activity of polysaccharide, with special reference to the effect of PS-K. A protein-bound polysaccharide isolated fromBasidiomycetes.Cancer and Chemother. 1:251–257 (in Japanese).
Tsukagoshi, S., and Ohashi, F. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouce sarcoma-180 and rat ascites hepatoma AH-13 by oral use.Gann. 65:557–558.
Usui, S., Urano, M., Koike, S., and Kobayashi, Y. 1976. Brief communication: effects of PS-K, a protein polysaccharide, on pulmonary metastase of C3H mouce squamous cell carcinoma.J. Natl. Cancer Inst. 56:185–187.
Vormittag, W. 1974. Zytostatische immunodepressive Therapie, chromosomal Aberrationen und karzinogene Wirkung.Wiener klini. Wschr. 3:69–75.
Wegmann, W., Largiarder, F., and Scheitlin, W. 1971, Malignant Reticulose und Nierentransplantation.Schweiz. med. Wschr. 101:80–84.
Zeuthen, E., and Friedrich, U. 1971. Chromosomenuntersuchungen bei Kindern von Imuranbehandelten Eltern.Humangenetik 12:74–76.
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Torigoe, K. Sister chromatid exchanges in children treated with azathioprine and cyclophosphamide, and effect of protein-bound polysaccharide (PS-K). Jap J Human Genet 25, 15–21 (1980). https://doi.org/10.1007/BF01876541
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DOI: https://doi.org/10.1007/BF01876541
