Abstract
Comparative genomic hybridization (CGH) analyses have detected gains of copy number on 13q, especially at 13q31-q32, in cell lines and primary cases of various types of lymphoma. Since amplification of chromosomal DNA is one of the mechanisms that can activate tumor-associated genes, and because 13q amplification had been reported in various other types of tumors as well, we attempted to define by fluorescence in situ hybridization (FISH) a common region at 13q31-q32 in which to explore genes that might be targets for the amplification events. Although the commonly amplified region we defined was relatively large (approximately 4 Mb), only one true gene, GPC5, was found there. GPC5 was over-expressed in lymphoma cell lines that had shown amplification, in comparison with those that had not. Our findings suggest that GPC5 is a likely target for amplification, and that over-expression of this gene may contribute to development and/or progression of lymphomas and other tumors.
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Acknowledgements
We thank Dr. Masao Seto (Aichi Cancer Center) for giving us information of CGH data in lymphoma cell lines used in the present study, and also thank Professor Yusuke Nakamura (Institute of Medical Science, The University of Tokyo) for his continuous encouragement. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology, and by Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation.
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Yu, W., Inoue, J., Imoto, I. et al. GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. J Hum Genet 48, 331–335 (2003). https://doi.org/10.1007/s10038-003-0026-2
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DOI: https://doi.org/10.1007/s10038-003-0026-2
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