Abstract
The mouse Dlx5 gene encodes a distal-less-related DNA-binding homeobox protein first expressed during early embryonic development in anterior regions of mouse embryo and is located on chromosome 6, which is the syntenic region to the human chromosome 7q21–q31 imprinting cluster. Recently, its human homologue, DLX5, was identified to be imprinted and maternally expressed, at least in normal human lymphoblasts and in brain tissues. In our study, we analyzed the imprinting status of mouse Dlx5 by RT-PCR, first in the F1 of a reciprocal cross between two different mouse strains, and second in heterozygous Dlx5 mutant mice. Both approaches revealed that mouse Dlx5 followed a biallelic pattern of expression in brain tissue and in testis. Our findings suggest that the Dlx5 gene escapes genomic imprinting, at least in mice of certain genetic backgrounds.
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Acknowledgements
We thank Dr. Haruaki Ninomiya, Dr. Kazuhiko Nakabayashi, and Dr. Akihiro Kurimasa for the valuable discussions and Ms. Candice Ginn T. Tahimic for critical reading and editing of this manuscript. YK is a research fellow of the Japan Society for the Promotion of Science. This study was partly supported by grants from the Ministry of Education, Sports, Science, and Technology and Ministry of Health, Labor, and Welfare of Japan.
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Kimura, M.I., Kazuki, Y., Kashiwagi, A. et al. Dlx5, the mouse homologue of the human-imprinted DLX5 gene, is biallelically expressed in the mouse brain. J Hum Genet 49, 273–277 (2004). https://doi.org/10.1007/s10038-004-0139-2
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DOI: https://doi.org/10.1007/s10038-004-0139-2
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