Abstract
Uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate, is important in the pyrimidine salvage pathway and is an attractive target for rational drug design by incorporation of prodrugs that are lethal to many parasitic organisms specifically. So far, uracil phosphoribosyltransferase has been reported in Arabidopsis thaliana only, not in mammals. In this study, a novel uracil phosphoribosyltransferase family cDNA encoding a 309 amino acid protein with a putative uracil phosphoribosyltransferase domain was isolated from the human fetal brain library. It was named human UPRTase (uracil phosphoribosyltransferase). The ORF of human UPRTase gene was cloned into pQE30 and expressed in Escherichia coli M15. The protein was purified by Ni-NTA affinity chromatography, but UPRTase activity could not be detected by spectrophotometry. RT-PCR analysis showed that human UPRTase was strongly expressed in blood leukocytes, liver, spleen, and thymus, with lower levels of expression in the prostate, heart, brain, lung, and skeletal muscle. Subcellular location of UPRTase–EGFP fusion protein revealed that human UPRTase was distributed in the nucleus and cytoplasm of AD293 cells. Evolutional tree analyses of UPRTases or UPRTase-domain-containing proteins showed that UPRTase was conserved in organisms. UPRTases of archaebacteria or eubacterium had UPRTase activity whereas those higher than Caenorhabditis elegans, which lacked two amino acids in the uracil-binding region, had no UPRTase activity. This means that human UPRTase may have enzymatic activity with another, unknown, factor or have other activity in pyrimidine metabolism.
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Abbreviations
- UPRTase:
-
Uracil phosphoribosyltransferase
- GFP:
-
Green fluorescent protein
- MTC:
-
Multiple tissue cDNA
- UMP:
-
Uridine monophosphate
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Acknowledgments
We thank Dr Tonghai Dou and Dr Wenbo Yu of Fudan University for helpful suggestions in relation to this paper. This work was supported by funds from the National Science Foundation of China (Grant Number 10490190 and 10490193).
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Jixi Li and Shengdong Huang contributed equally to this work.
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Li, J., Huang, S., Chen, J. et al. Identification and characterization of human uracil phosphoribosyltransferase (UPRTase). J Hum Genet 52, 415–422 (2007). https://doi.org/10.1007/s10038-007-0129-2
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DOI: https://doi.org/10.1007/s10038-007-0129-2
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