Abstract
In the candidate gene approach, information about the distribution of single nucleotide polymorphisms (SNPs) is a crucial requirement for choosing efficient markers necessary for a case-control association study. To obtain such information, we discovered SNPs in 13 genes related to atherosclerosis by resequencing exon-flanking regions of 32 healthy Thai individuals. In total, 194 polymorphisms were identified, 184 of them SNPs, four insertions, and the rest deletions. Fifty-nine of the SNPs were characterized as novel polymorphisms, and these accounted for 30% of the identified SNPs. Comparing allele frequency distributions of the Thai population with other Asian populations shows similar patterns. In contrast, a low correlation pattern (r = 0.521) was found when comparing with either Caucasian or African populations. However, some rare alleles (rs11574541 and rs10874913) are found in the Thai population but not in other Asian populations. Most of the novel SNPs found were located outside the haplotype blocks generated by known SNPs in the Thai population. Only 5.77% of the novel SNPs lies in these defined haplotype blocks. The selection of haplotype-tagging SNPs shows that 8 of 13 genes benefited from the ethnic-specific genotype information. That is, when at least one novel SNP was present, the tagging SNPs chosen were altered. Functional prediction of 16 nonsynonymous SNPs (nsSNPs) by three different algorithm tools demonstrated that five nsSNPs possibly alter their corresponding protein functions. These results provide necessary information for conducting further genetic association studies involving the Thai population and demonstrate that resequencing of candidate genes provides more complete information for full genetic studies.
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Acknowledgments
We thank Justin Dantzer for designing figures used in this article and Lang Li and Jeesun Jung for helpful advice. CT received the postdoctoral fellowship from the National Center for Genetic Engineering and Biotechnology, Thailand, under the Thailand SNP Discovery Project. SP is funded by Thailand Center for Excellence in Life Sciences (TCELS) under the Postdoctoral Scholarships Program. SDM is funded by NIH K22LM009135.
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Chintana Tocharoentanaphol and Somying Promso contributed equally to this work.
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Tocharoentanaphol, C., Promso, S., Zelenika, D. et al. Evaluation of resequencing on number of tag SNPs of 13 atherosclerosis-related genes in Thai population. J Hum Genet 53, 74–86 (2008). https://doi.org/10.1007/s10038-007-0220-8
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DOI: https://doi.org/10.1007/s10038-007-0220-8
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