Abstract
Allelic mutations of the lysosomal β-galactosidase gene cause heterogeneous clinical phenotypes, such as GM1 gangliosidosis and Morquio B disease, the former being further classified into three variants, namely infantile, juvenile and adult forms; and heterogeneous biochemical phenotypes were shown in these forms. We tried to elucidate the bases of these diseases from a structural viewpoint. We first constructed a three-dimensional structural model of human β-galactosidase by means of homology modeling. The human β-galactosidase consists of three domains, such as, a TIM barrel fold domain, which functions as a catalytic domain, and two galactose-binding domain-like fold domains. We then constructed structural models of representative mutant β-galactosidase proteins (G123R, R201C, I51T and Y83H) and predicted the structural change associated with each phenotype by calculating the number of affected atoms, determining the root-mean-square deviation and the solvent-accessible surface area, and by color imaging. The results show that there is a good correlation between the structural changes caused by amino-acid substitutions in the β-galactosidase molecule, as well as biochemical and clinical phenotypes in these representative cases. Protein structural study is useful for elucidating the bases of these diseases.
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Acknowledgements
We thank Jay Ponder and colleagues (Washington University) for providing us with the TINKER software. This work was partly supported by grants from the Japan Society for the Promotion of Science, the High-Technology Research Project of the Ministry of Education, Science, Sports and Culture of Japan, the Ministry of Health, Labor and Welfare of Japan, the Japan Science and Technology Agency and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation.
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Morita, M., Saito, S., Ikeda, K. et al. Structural bases of GM1 gangliosidosis and Morquio B disease. J Hum Genet 54, 510–515 (2009). https://doi.org/10.1038/jhg.2009.70
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DOI: https://doi.org/10.1038/jhg.2009.70
