Abstract
Gene associated with Retinoid-Interferon-induced Mortality (GRIM)-19 was originally identified as a regulatory gene necessary for interferon-β and retinoic acid-induced cell death. Further studies revealed that GRIM-19 is a subunit of mitochondrial respiratory chain complex I. Previous studies show that the expression of GRIM-19 is lost or severely depressed in a number of primary renal cell carcinomas (RCCs) and in some urinogenital tumors. Four point mutations were found in the GRIM-19 gene in mitochondria-rich (Hürthle) tumors and one point mutation was reported in RCC. In this study, we report an alternatively splicing form of GRIM-19 mRNA with intron 3 by reverse transcriptase PCR. This splicing variant is found in kidney tumor tissues but not in matched normal tissues. Furthermore, we found that in addition to GRIM-19, the protein level of NDUFS3, which is another mitochondrial complex I subunit, was also diminished in kidney tumor tissues when compared with paired normal tissues. Our finding suggested that the alternative splicing form of GRIM-19 is tumor tissue specific.
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This work was supported by the Agency for Science, Technology and Research of Singapore.
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He, X., Cao, X. Identification of alternatively spliced GRIM-19 mRNA in kidney cancer tissues. J Hum Genet 55, 507–511 (2010). https://doi.org/10.1038/jhg.2010.57
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DOI: https://doi.org/10.1038/jhg.2010.57
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