Figure 4 | Journal of Human Genetics

Figure 4

From: Truncated UDP-glucuronosyltransferase (UGT) from a Crigler–Najjar syndrome type II patient colocalizes with intact UGT in the endoplasmic reticulum

Figure 4

Subcellular localization of EGFP-UGT1A1 and UGT1A1-p.Q331X-mKate2 in 293T cells. Subcellular localization of intact and truncated UGT1A1 determined by fractionating cell lysates from 293T cells expressing EGFP-UGT1A1 or UBT1A1-p.Q331X-mKate2, or both. Whole-cell extracts (W), pellets (P) and soluble cytosolic fractions (C) were analyzed using anti-UGT1A1, anti-GFP or anti-tRFP antibodies. Anti-H3, anti-Calnexin and anti-Tubulin were used as positive control markers for the microsomal and cytosolic fractions.

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