Figure 5 | Journal of Human Genetics

Figure 5

From: Truncated UDP-glucuronosyltransferase (UGT) from a Crigler–Najjar syndrome type II patient colocalizes with intact UGT in the endoplasmic reticulum

Figure 5

Fluorescence photobleaching of EGFP, EGFP-UGT1A1 and UGT1A1-p.Q331X-Venus. HEK293T cells expressing EGFP (a), EGFP-UGT1A1 (b) or UGT1A1-p.Q331X-Venus (c) were subjected to photobleaching and the fluorescence recovery after photobleaching recoveries were determined. Figures show the mobility of the EGFP, EGFP-UGT1A1 or UGT1A1-p.Q331X-Venus molecules. Images were captured using an LSM5 33 EXCITER confocal microscope (Carl Zeiss). Excitation was performed at 488 (514) nm to visualize EGFP (Venus) using LP505 (530) nm emission filters. Fluorescence recovery after photobleaching was performed using the region of interest, 2 μm in diameter, and four bleaching iterations. Graphic imaging was processed with the Zen software. A full color version of this figure is available at the Journal of Human Genetics journal online.

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