Table 1 Protocol for estrous-cycle monitoring in Sprague-Dawley rats

From: Effects of tribromoethanol-medetomidine combination on cycling and ovariectomized Sprague-Dawley rats

Day(s)a

 

Protocol

0

 

Arrival of the animals

1–8

Study 1

Estrous cycle monitoring

8

 

Study 1: Testing TBE-MT anesthetic

9–15

 

Estrous cycle monitoring

15

Study 2

Surgical procedure: Group 2 was ovariectomized

16–22

 

Estrous cycle monitoring

22

 

Study 2: Testing TBE-MT anesthetic

  1. aStudy 1 was from day 1 through day 15. The same animals were used in the second study from day 15 through day 22