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Simplified Surgical Placement and Stabilization Methods for Intracerebroventricular Cannulas in Rat Lateral Ventricles

Lab Animal volume 32, pages 43–48 (2003)Cite this article

Abstract

Intracerebroventricular cannulation in rat models is an efficient tool for exploring the effects of substances directly injected into the CNS, bypassing the blood–brain barrier. Techniques for surgically securing the ICV cannula require a balance between ease of application and adequate stability. The authors tested several methods of lateral ventricle cannula stabilization, especially focusing on a comparison of cyanoacrylate gel to cranioplastic cement with an anchoring bone screw.

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Figure 1: Location of bregma and stabilization of cannula with cyanoacrylate gel.
Figure 2: Cannula stabilization with cranioplastic cement and anchoring screw.
Figure 3: Closure after cannula placement and staining of ventricles.
Figure 4: Postoperative food intake and body weight.

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Acknowledgements

We are grateful for the assistance of Allen Mikhail and Evan Ong in surgical photography.

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Authors and Affiliations

  1. Jho and Espat are affiliated with the Department of Surgery, University of Illinois at Chicago.,

    David H. Jho BA & N. Joseph Espat MD, MS, FACS

  2. Engelhard and Juarez are affiliated with the Department of Neurosurgery, University of Illinois at Chicago.,

    Herbert H. Engelhard MD, PhD, FACS & Altair Juarez BS

Authors
  1. David H. Jho BA
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  2. Herbert H. Engelhard MD, PhD, FACS
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  3. Altair Juarez BS
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  4. N. Joseph Espat MD, MS, FACS
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Corresponding author

Correspondence to N. Joseph Espat MD, MS, FACS.

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Jho, D., Engelhard, H., Juarez, A. et al. Simplified Surgical Placement and Stabilization Methods for Intracerebroventricular Cannulas in Rat Lateral Ventricles. Lab Anim 32, 43–48 (2003). https://doi.org/10.1038/laban1003-43

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