Figure 3 | Laboratory Investigation

Figure 3

From: Matrix metalloproteinase 7 is required for tumor formation, but dispensable for invasion and fibrosis in SMAD4-deficient intestinal adenocarcinomas

Figure 3

Intestinal tumor invasion in the cis-Apc/Smad4 Mmp7−/− tumors. (a) The invasion depths of the polyps (Φ ≥2 mm) in the cis-Apc/Smad4 and cis-Apc/Smad4 Mmp7−/− mice (n=50 tumors from three mice per group). The polyps stained with H and E were classified according to their invasion depth, ie, mucosa (m), submucosa (sm), muscularis propria (mp) and serosa (se). Results are presented as proportions among the polyps examined (mean±s.d. from 50 polyps in each strain). (b) Expression of MMP mRNAs determined by RT-PCR. Total RNA was prepared from the intestinal tissues of wild-type mice or ApcΔ716, cis-Apc/Smad4 and cis-Apc/Smad4 Mmp7−/− polyps. (c) Invasion front of the cis-Apc/Smad4 (left) and cis-Apc/Smad4 Mmp7−/− (right) polyps. Serial sections were double stained for pan-cytokeratin (green) and CD11b (red) or CD34 (green) and CD31 (red), respectively. Scale bars show 100 μm.

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