Figure 5 | Laboratory Investigation

Figure 5

From: Matrix metalloproteinase 7 is required for tumor formation, but dispensable for invasion and fibrosis in SMAD4-deficient intestinal adenocarcinomas

Figure 5

Expression of collagen type-I in the cis-Apc/Smad4 Mmp7−/− tumors. (a) The intestinal polyps of the ApcΔ716 (left), cis-Apc/Smad4 (middle) and cis-Apc/Smad4 Mmp7−/− (right) stained for collagen type-I. Insets show higher magnifications of the boxed areas. Scale bars show 200 and 100 μm (inset). (b) Expression of collagen type-I mRNA determined by RT-PCR. Col1a1 and Col1a2 show α1 and α2 chains of collagen type-I, respectively. Total RNA was prepared from the intestinal tissues of wild-type mice or ApcΔ716 and cis-Apc/Smad4 polyps. (c) Expression of collagen type-I mRNA in the intestinal polyps (P) and normal tissues (N) from cis-Apc/Smad4 (+/+) and cis-Apc/Smad4 Mmp7−/− (−/−) mice.

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