Figure 2

Real-time PCR of selected genes in an expanded patient cohort. We analyzed the RNA expression of HIF-1α, HIF-2α, VEGF-A, and p21 in patients with available microarray results as well as in an expanded patient cohort (Table 1). Microarray expression data are shown as ratio to the reference RNA (left column). Real-time PCR expression values are shown as ratios to the respective housekeeper gene (GAPDH and cyclophilin A, right columns). Error bars represent the standard deviation of the mean. SD: stable disease, PD: progressive disease. The P-value was calculated using a two-sided t-test with a CI of 95%.