Figure 2
Organization of the high-resolution mapping studies of 13q14 region. The high-resolution mapping studies of 26.9 Mb in the 13q14 region containing RB1 were performed with SNP markers using WOHGM strategy. The frequency of involvement of a 1.34-Mb minimal deleted region around RB1 was confirmed on 111 samples of bladder tumors by allelotyping of 100 SNPs mapping across 3.16-Mb segment around RB1. The mapping studies were followed by the genomic content analysis of the minimal deleted region, as well as expression, sequencing, methylation, and in vitro functional studies of its positional candidate FR genes. Some of the studies outlined here were previously published16 and are complemented with the new analyses included in this paper.
