Figure 7
Increased lymphocyte infiltration and expression of inflammatory cytokines in Muc2/IL-10DKO mice. (a) Representative sections presenting lymphocyte infiltration into the distal colon of WT, IL-10−/−, Muc2−/− and Muc2/IL-10DKO mice detected using an anti-CD3 antibody. Arrows in the first three panels indicate presence of CD3+ cells. (b) Real-time PCR data showed a significant upregulation of the inflammatory cytokines TNF-α, IL-1β and IL-6 (P=0.02) in the distal colon of Muc2/IL-10DKO mice (dotted bars) compared to WT mice whereas no significant changes were detected in both IL-10−/− (black bars) and Muc2−/− (white bars) mice, compared to WT mice, 5 weeks of age. (c) Analysis of the serum showed an increase in expression of inflammatory cytokines IL-12p70, TNF-α, IFN-γ, MIP-1 and IL-6 in the serum of Muc2/IL-10DKO mice (dotted bars) compared to WT mice (striped bars). Muc2/IL-10DKO, n=7; WT, n=4; error bars indicate the s.e.m.
