Figure 5 | Laboratory Investigation

Figure 5

From: Potential utility of xenobiotic mitogens in the context of liver regeneration in the elderly and living-related transplantation

Figure 5

(a) Representative photomicrographs, which illustrate the effect of TCPOBOP-induced liver enlargement on the regenerative response, subsequent to two-third PH, in young mice. TCPOBOP (3 mg/kg) was given 3 days before PH. At this time point, mice were subjected to 70% PH and killed 72 h later. A group of mice was subjected to PH in the absence of TCPOBOP pretreatment. All animals were given BrdU (1 mg/ml) in drinking water until the time of killing (× 200, sections counterstained with hematoxylin). TCP, TCPOBOP-treated mice; (b) western blot analysis of cell cycle-associated proteins. Extracts for cyclin D1, PCNA and E2F (100–150 mg/lane) were prepared from the livers, and western analysis was performed as described in Materials and Methods. Albumin was used as a loading control. Each lane represents an individual sample. TCP, TCPOBOP-treated mice; CO, controls.

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