Figure 6

Immunohistochemical double staining of the labyrinths with antibodies against the viral antigen and antibodies specific to myeloperoxidase (MPO) (a), factor VIII (b), or neuronal nuclei (NeuN) (c) in the MCMV-i.p.-infected mice with LPS. (a) Sections were reacted with anti-IE1 mAb, incubated with secondary Ab, and colored with AEC, then reacted with anti-MPO pAb and incubated with ALP-conjugated secondary Ab, colored with Fast Blue. M, mesenchymal region; SV, scala vestibuli. (b) Sections were reacted with anti-IE1 mAb, incubated with secondary Ab, and colored with AEC, then reacted with anti-factor VIII pAb and incubated with ALP-conjugated secondary Ab and colored with Fast Blue. Arrows show double-stained cells. ST, scala tympani. (c) Sections were reacted with anti-Q3 (cytoplasmic antigen) mAb, incubated with secondary Ab, and colored with AEC, then sections were reacted with anti-NeuN mAb and incubated with ALP-conjugated secondary Ab and colored with Fast Blue. SG, spiral ganglia; SM, scala media.