Figure 2
From: Stat4 suppresses the proliferation of connective tissue-type mast cells
Increased number and enhanced cell cycle progression in cultured CTMCs derived from Stat4-deficient mice. (a) The cell numbers of cultured splenocytes derived from Stat4+/+ or Stat4−/− Balb/c mice in KitL-contained medium with or without STI571 (Stat4+/+ mice; n=5, Stat4−/− mice; n=7) *P<0.05 by t-test when compared to the value of Stat4−/− Balb/c CTMCs to that of cultured Stat4+/+ CTMCs. (b) Cell cycle analyses of cultured CTMCs derived from Stat4+/+ or Stat4−/− Balb/c mice were conducted. Data are representative of 11 individual experiments. The gate referred to as ‘M1’ refers to G2/M and S phases. (c) Incorporation of bromodeoxyuridine (BrdU) into cultured MMCs or CTMCs derived from Stat4+/+ or Stat4−/− Balb/c mice. *P<0.05 by t-test when compared to the value of cultured Stat4+/+ MMCs. #P<0.05 by t-test when compared to the value of cultured Stat4+/+ CTMCs.
