Figure 4 | Laboratory Investigation

Figure 4

From: Expression of specific hepatocyte and cholangiocyte transcription factors in human liver disease and embryonic development

Figure 4

Expression of hepatocyte-associated transcription factors in biliary epithelial cell (BEC) in different hepatic diseases. (a–i) Immunohistochemical staining of HNF4α, HNF6, and HepPar1 in normal vs massive hepatic necrosis and end-stage HCV cirrhotic liver. BEC in normal adult liver are negative for HNF4α (a) and HNF6 (d). BEC in massive hepatic necrosis (MHN) express HNF4α (b) and HNF6 (e). BEC also expresses HNF6 in MHN (c) and HCV (f). The appearance of the single-gene hepatocyte marker HEPAR is also prominent is many ductular cells. Arrows indicate positive staining. BD, bile duct; CV, central vein; PV, portal vein. (j) Quantitative analysis of HNF4α immunostaining in liver disease in hepatocytes vs BEC. (k) Quantitative analysis of HNF6 immunostaining in liver disease in hepatocytes vs BEC. Both HNF4α and HNF6 emerge in BECs in diseases associated with chronic hepatocyte injury. In both (j and k), the bars indicate the mean and standard error from cell counts of separate cases, each disease from a number of separate specimens as described in ‘Materials and Methods’. Blue, hepatocytes; red, biliary cells.

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