Figure 1 | Laboratory Investigation

Figure 1

From: Classification of renal cell carcinoma based on expression of VEGF and VEGF receptors in both tumor cells and endothelial cells

Figure 1

Localization and quantitation of VEGF-R3 in different tumor elements of an RCC histospot. (a) A cocktail of anticytokeratin and streptavidin was used to create a tumor cell mask. (b) VEGF-R3 expression in the tumor mask (green pixels) was distinguished from expression from other elements in the stroma (red pixels) using AQUA. AQUA then quantitates the VEGF-R3 expression in the tumor by summing the total intensity of VEGF-R3 expression in the mask divided by the number of masked pixels (ie, the sum of green pixel intensity/the number of green pixels). (c) An antibody against CD34 was used to create a tumor vessel mask. (d) VEGF-R3 expression in the vessels (green) is then independently assessed using the method in panel b.

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