Figure 4
From: Changes in S-adenosylmethionine and GSH homeostasis during endotoxemia in mice
Hepatic GNMT mRNA and protein levels during endotoxemia. (a) Steady-state mRNA levels of GNMT were determined by northern blot analysis as described in ‘Materials and Methods’. Membranes were sequentially probed with GNMT and 18S rRNA. (b) Summary of results expressed as % of control GNMT mRNA levels as determined by both northern blot analysis and real-time PCR. SAMe pretreatment group is labeled SAMe+LPS and SAMe post-treatment group is labeled LPS+SAMe. (c) Steady-state protein levels of GNMT were determined by western blot analysis and densitometric values are shown below the western blot. *P<0.005 vs control group, †P<0.01 vs control group, n=4–8 per group.
