Figure 1
From: Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects
Basal activity of early osteogenic marker ALP in human osteosarcoma cells. (a) Quantitative analysis of basal ALP activity in four commonly used OS lines and control mouse mesenchymal progenitor line C3H10T1/2. Subconfluent cells were cultured at the indicated serum conditions for 36 h and collected for ALP assay as described in ‘Materials and Methods’. Each assay condition was done in duplicate. (b) Qualitative analysis of basal ALP activity in four OS lines. OS cells were cultured in 1% FCS for 36 h and stained histochemically for ALP activity as described in ‘Materials and Methods’. (c) Basal ALP activity in primary OS lines derived from patients. Twelve primary OS tumor cell lines, primary human marrow stromal cells (hMSC), and immortalized human osteoblast line hFOB were cultured in 1% FCS for 36 h and collected for ALP assays. SaOS2 was included as a control of high basal ALP activity. See ‘Materials and Methods’.
