Figure 5
From: Pancreatic duodenal homeobox 1 protein is a novel β-cell-specific autoantigen for type I diabetes
Antigen-specific T-cell proliferation to Pdx1 and its truncated forms. Splenocytes (106 cells/well) isolated from a NOD mouse with new-onset diabetes were incubated in a 96-well plate. Pdx1 and various truncated proteins were added into the wells at a final concentration of 1 μg/ml for 48 h, following by adding [3H]thymidine for an additional 24 h. Cells were captured on filter paper, washed five times, and [3H]thymidine incorporation was determined by scintillation counting. Background count is without [3H]thymidine. **P<0.01 and ***P<0.001 (Student's t-test). SI, stimulation index.
