Figure 1 | Laboratory Investigation

Figure 1

From: Small peptide inhibitor of JNKs protects against MPTP-induced nigral dopaminergic injury via inhibiting the JNK-signaling pathway

Figure 1

Effects of Tat peptides on the phosphorylation of JNK3 and JNK1/2. (a and b) Fluorescence can be observed in SNc of mice subjected cerebral ventricular injection of Tat-JBD-dansyl and Tat-JBD-4A-dansyl, whereas the same region showed only background signal in the mice treated with saline. a and b: × 40; scale bar in d=200 μm. (c and e) Effects of Tat peptides on the phosphorylation of JNK3 in SNc. Bands corresponding to JNK3 and p-JNK3 were scanned and the intensities were represented as folds vs saline animals. (d and f) Effects of Tat peptides on the phosphorylation of JNK1/2 in SNc fraction. Bands corresponding to JNK1/2 and p-JNK1/2 were scanned and the intensities were represented as folds vs saline groups. Phosphorylation of JNK3 was examined by immunoprecipitation (IP) with anti-p-JNKs antibody followed by immunoblotting (IB) with the antibody against JNK3. Phosphorylation of JNK1/2 was examined by IB with anti-p-JNK1/2. The protein levels of JNK3 and JNK1/2 were examined by immunoblotting with anti-JNK3 and anti-JNK1/2 antibodies. Data are expressed as the mean±s.d. and as folds vs respective saline groups. aP<0.05 vs saline; bP<0.05 vs MPTP groups (n=6 mice).

Back to article page