Figure 7
Effects of Tat-JBD on cell death and survival in SNc. (a) Tat–JBD attenuated MPTP-induced DA terminal loss in striatum and dopaminergic neuronal death in the SNc. TH-stained sections of SNc in saline group (a-A), mice subjected MPTP-induced lesion (a-B), administration of the Tat-JBD-4A (a–C) and Tat-JBD (a–D) following MPTP injection. When compared to Tat-JBD treated mice (a-D1), Tat-JBD-4A-treated mice following MPTP lesion showed marked reduction in TH-positive fibers in striatum (a-C1). (a-A1, a-B1) TH staining in striatum of saline and MPTP-treated mice. Scale bars: A, B, C, D=200 μm; (A1, B1, C1, D1)=400 μm. (c) Quantitative analysis of the protective effects of Tat-JBD against MPTP-induced mice model of nigrostriatal damage. aP<0.05 vs saline control bP<0.05 vs MPTP injection groups. (b) Representative SNc photomicrographs of TUNEL staining counterstained with methyl green were shown. Mice were subjected to saline (b-A, b-A2) and MPTP (b-B, b-B2), and mice subjected to MPTP with administration of 100 μg/4 μl of Tat-JBD-4A (b-C, b-C2) or 100 μg/4 μl of Tat-JBD (b-D, b-D2) for 5 days after i.p. MPTP. Some cells had shrunken, darkly stained nuclei and apoptotic bodies (arrowheads). Others had lightly stained, large and swollen nuclei. Boxed areas in left column are shown at higher magnification in right column. A, B, C, D: × 40; A2, B2, C2, D2: × 400. Scale bar in D=200 μm; scale bar in D2=10 μm. (d) Quantitative analysis of the protective effects of Tat-JBD against MPTP-induced mice model of nigrostriatal damage. aP<0.05 vs saline control; bP<0.05 vs MPTP-injection groups.
