Figure 2
From: Runx2 regulates survivin expression in prostate cancer cells
In vivo occupancy of the human survivin gene promoter by Runx2 in C4-2B cells cultured in full serum medium. (a) A line diagram of the survivin promoter (−2.8 kb) indicating the positions of the various potential Runx2-binding sites. The sequence is numbered relative to the ATG translation initiation site and each site is marked by the number of the first nucleotide. The number in the parentheses indicates the weighted score of sequence conservation at that site calculated.12 Underscored region-I and II were analyzed by ChIP assays for which a region marked as Ctrl served as negative control for the binding assays. The nucleotide sequence encompassing region-I and II is presented underscoring the theoretical Runx- and Smad-interacting elements. Runx sites are indicated in bold and underlined with the score of conservation in the parenthesis. The unmatched nucleotide is italicized. Smad elements,29, 34 in italics, are also underlined. (b) Detection of Runx2 binding to region-I and II by the cross-linking ChIP assay. The immunoprecipitates were subjected to PCR analysis to determine the binding affinity. (c) Detection of Runx2 binding to region-I by native ChIP assay.
