Figure 4

Double immunostaining of IL-22 and CD3 (a), IL-17 (b), CD56 (c), or CD11c (d) in UC mucosa (left, IL-22; right, other markers; middle, merged image). IL-22- and IL-17-positive cells were mainly observed in the lamina propria near muscularis mucosa in UC tissues (green signal in A and red signal in B, respectively). Some IL-22-positive cells co-expressed CD3 or IL-17 there (yellow signal in A and B, respectively). (c) Inflammatory cells partially co-expressed IL-22 and CD56 in the lamina propria near muscularis mucosae in UC tissue. (d) IL-22-positive cells were hardly positive for CD11c in such portion. Nuclei were stained with DAPI (blue signal) using ProLong Gold and SlowFade Reagents (Invitrogen). E, epithelial cell structure.