Figure 3

Liver semi-thin sections of control and DENV-2-infected mice, stained with methylene blue and azure II solution. (a) Liver parenchyma from non-infected mice with normal aspect (bar=2 μm). (b) Liver of one mouse killed 2 days p.i. showing hepatocytes with large lipid droplets (black stars) in the periportal area (bar=6 μm). (c) Damages observed 7 days p.i. presenting heterogeneous parenchyma, which consists of dark and compact hepatocytes flanked by clear and swollen (asterisks) or regenerated cells (bar=3 μm). (d) Sections obtained 14 days p.i. showing more extensive areas of large lipid droplets (black stars) and plasma leakage (PL). (e) Section observed 49 days p.i. presenting binucleated hepatocytes with speckled cytoplasm (white star) (bar=2 μm). Ly, Lymphocytes, KC, Kupffer cells.